RB 2X qPCR complete Master Mix (SYBR+ROX)
RB qPCR Complete Master Mix is a ready to use 2X concentrated solution is optimized for dye based qPCR reaction on a real-time PCR instrument. It contains a high performance Hot-Start Taq DNA Polymerase, dNTPs, MgCl2, SYBR Green I dye, enhancers, stabilizers, and a passive reference dye(ROX). It doesn’t contain primers or a DNA template. This qPCR Master mix is specifically designed for routine Real Time PCR applications.
Pricing
| Catalog | Description | Size | Price |
|---|---|---|---|
| RB23081 | RB qPCR Complete Master Mix (SYBR+ROX) – 20µL x 100 Rxn | 1 mL | ₹ 3,000 |
| RB23082 | RB qPCR Complete Master Mix (SYBR+ROX) – 20µL x 500 Rxn | 5 x 1 mL | ₹ 12,000 |
| RB23082 | RB qPCR Complete Master Mix (SYBR+ROX) – 20µL x 500 Rxn | 5 mL | ₹ 12,000 |
| RB23082 | RB qPCR Complete Master Mix (SYBR+ROX) – 20µL x 2500 Rxn | 5 x 5 mL | ₹ 55,000 |
Pack Contents
RB qPCR Complete Master Mix (SYBR+ROX)
RB 2x qPCR Complete Master Mix, is a ready-to-use solution optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument. This master mix includes antibody-mediated RB Taq DNA polymerase, dNTPs, MgCl₂, SYBR Green I dye, and a blend of passive reference dyes, along with specialized enhancers and stabilizers for superior performance and consistency.
Applications
- Gene Expression Analysis
- Pathogen Detection
- Genotyping and Mutation Screening
- Precise Quantification of DNA/RNA
Instrument Compatibility
RB SYBR qPCR Complete Master Mix is compatible with Real-time PCR instruments that uses ROX as a reference dye such as all Bio-Rad and ROX dependent Applied Biosystems real-time PCR instruments.
The products are intended solely for research purposes and should not be utilized for diagnostic assays.
- Store at -20°C in a constant temperature freezer
- Avoid exposure to direct light
Reaction setup and Thermal cycling Protocol
- Thaw RB qPCR Complete Master Mix and reaction components on ice, mix thoroughly and briefly spin to collect solutions at the bottom of the tubes and avoid exposure to light.
- Prepare the reaction mix by adding all the required components according to the following recommendations (Table 1).
| Table 1. Rection setup | |||
| Components | Volume per 20µl Reaction | Volume per 10µl Reaction | Final concentration |
| RBTM SYBR qPCR Master Mix (2X) | 10 µl | 5 µl | 1X |
| Forward Primer (10µM) | 1 | 0.5 | 0.1 -1 µM |
| Reverse Primer (10µM) | 1 | 0.5 | 0.1 -1 µM |
| Template DNA-cDNA | 1 | 0.5 | <100ng cDNA, < 250ng of genomic DNA |
| Nuclease free water | Upto 20 µl | Upto 10 µl | – |
*Proportionally scale up all components based on the number of samples and reaction volumes
| Table 2. Thermal Cycling Protocol | |||
| Step | Temperature | Time | Cycle |
| Initial Denaturation | 95°C | 1-2 min | 1 |
| Denaturation | 95°C | 5-10 sec | 35-40 |
| Annealing/ Extension | 60°C to 65°C | 20-30 sec | |
| Melt Curve Analysis | 65°C to 95°C | Variable | – |
Related Products
RB 2x PCR Master Mix with dNTPS and buffer
Cat # : RB23021
2,900 / 100 Rxn