RB cDNA Synthesis Kit
The RB cDNA Synthesis Kit features a modified Moloney Murine Leukemia Virus (MMLV) reverse transcriptase, which is optimized for synthesizing high-quality cDNA from a wide variety of RNA templates. This reverse transcriptase ensures efficient reverse transcription, even from challenging RNA sources. The kit includes a 5X Reaction Mix, which contains oligo(dT) primers, random hexamers, dNTPs, Mg²⁺, an RNase inhibitor, and stabilizers, all carefully formulated to promote robust and accurate cDNA synthesis. This comprehensive mixture enables reliable cDNA synthesis with minimal RNA degradation and ensures high yield and fidelity.
Pricing
| Catalog | Description | Size | Price |
|---|---|---|---|
| RB23051 | RB cDNA synthesis Kit with 5X reaction buffer contains dNTPS, random hexamers, Oligo (dT) | 50 rxn | ₹ 8,000 |
| RB23052 | RB cDNA synthesis Kit with 5X reaction buffer contains dNTPS, random hexamers, Oligo (dT) | 100 rxn | ₹ 12,000 |
| RB23053 | RB cDNA synthesis Kit with 5X reaction buffer contains dNTPS, random hexamers, Oligo (dT) | 5 x 100 rxn | ₹ 52,000 |
Pack Contents
RB Reverse transcriptase mix (200U/µL)
RB 5x cDNA Reaction mix
Nuclease free water
RB First strand cDNA synthesis kit features a modified Moloney murine leukemia virus (MMLV) reverse transcriptase, meticulously optimized to ensure cDNA synthesis from a diverse range of RNA templates. The distinctive combination of oligo(dT), random hexamer primers, dNTPs, Mg+2, RNase inhibitor and stabilizers within the RBTM cDNA reaction mix demonstrates exceptional performance with a wide variety of target molecules. Specifically tailored for target lengths from <1kb up to 2 kb, this kit stands as an ideal choice for reliable and efficient cDNA synthesis.
Bulk, custom, and OEM packs
For bulk, custom and OEM packs and glycerol enzyme, Please write to us – contact@ribogenbiosciences.com
The products are intended solely for research purposes and should not be utilized for diagnostic assays.
Store at -20°C in a constant temperature freezer
Reaction setup
For successful cDNA synthesis, good quality RNA is a key element. The quality of RNA can be assessed using agarose gel electrophoresis. The 5x RBTM cDNA Reaction mixture may contain some precipitate upon thawing; please ensure complete resuspension by mixing thoroughly and use it as directed in the table below.
| Components | Volume per Reaction (µL) |
| 5x RBTM cDNA Reaction Mix | 4 |
| RBTM Reverse transcriptase | 1 |
| Nuclease-free Water | Variable |
| RNA template (up to 1µg)* | Variable |
| Total Volume | 20 |
* For RNA template more than 1µg the reaction volumes can be scaled up (for example, when using 2µg of RNA template reaction volume can be scaled up to 40µL) to ensure optimum synthesis efficiency
Reaction Protocol
| Priming | 5 min at 25°C |
| Reverse transcription | 20 min at 46°C for < 1kb 60min at 46°C for up to 2kb |
| RT Inactivation | 2 min at 95°C |
| Hold (Optional) | 4°C |
“The Discovery and Impact of MMLV Reverse Transcriptase”
MMLV (Moloney Murine Leukemia Virus) reverse transcriptase was discovered through research on retroviruses, specifically the Moloney murine leukemia virus. This enzyme enables retroviruses to convert their RNA genomes into DNA, a critical step for replication. The discovery of MMLV reverse transcriptase has been essential for developing techniques like cDNA synthesis, advancing genetic research.
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